This article has been reviewed according to Science X's editorial process and policies. Editors have highlighted the following attributes while ensuring the content's credibility: In a new research report, scientists at Johns Hopkins Medicine say they have developed a technology that allows them to capture a 3D molecular "snapshot" of hundreds of hair follicles as they develop and then reconstruct the fourth dimension—time—to create a stop-motion animation of how this organ forms. The research was published in the journal Cell.
Hair follicles are the smallest, most numerous organs in our bodies, but they form in ways similar to those of other organs, the scientists say. This advancement paves the way for understanding how organ development may go awry in people with certain congenital conditions. "Our four-dimensional (4D) map of the hair follicle from mice serves as a model system for understanding broad-stroke fundamentals of how organs develop," says Reza Kalhor, Ph.D., associate professor of biomedical engineering at the Johns Hopkins University School of Medicine, who led the recent study.
By analyzing the 4D map, the researchers were able to identify distinct phases in this organ's formation—a complex choreography of thousands of cells. First, the precursor cells organize themselves in space to establish the organ's spatial axis perpendicular to the skin surface. Next, these precursors differentiate into the many cell types needed to form hair follicles.
Finally, these new cell types grow and morph into a mature follicle, preparing to produce the hair strand. The research further compares the hair follicles of normal mice with hairless mice lacking a gene, Foxn1, which is critical for hair growth. This comparison may help scientists understand how medical conditions that cause hair loss develop, which may lead to new ways to prevent and treat hair loss in the future, says Luis Garza, M.D., Ph.D., a professor of dermatology at the Johns Hopkins University School of Medicine and co-author of the paper.
Scientists have long sought a way to visualize how organs develop over time. Doing so may lead to a better understanding of how certain inherited conditions may affect organ development or to capture how and when tumors develop, and could lead to earlier diagnoses and treatments, Kalhor says. "This problem remains complicated because organs are generated by millions of cells in very complex, coordinated processes," says Soichiro Asami, the first author of the paper and a Ph.D. candidate in Kalhor's lab.
"We developed a technology that tackles the problem of visualizing this and applied it to hair follicles, because there are hundreds of them spanning every stage of organ development." Biomedical engineers Kalhor and Asami partnered with Garza, who frequently studies Foxn1 mice as a model for understanding how hair growth disorders develop and finding new ways to treat them. Garza provided key context and background on hair follicle biology and tissue samples from normal and hairless mice in this recent study. The researchers developed a new molecular imaging tool, 3D DNase-Enhanced Expression Profiling (3DEEP), that allows them to analyze pieces of tissue large enough to capture entire hair follicle organs in skin samples from normal mice and bald mice. 3DEEP removes genomic DNA from the skin samples, which can interfere with chemical reactions used to visualize fragile pieces of messenger RNA essential for understanding organ development.
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